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KMID : 1007520190280061819
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Food Science and Biotechnology
2019 Volume.28 No. 6 p.1819 ~ p.1828
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Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes
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Kang Bo-Bin
Kim Chae-Young
Hwang Ji-Su
Sun So-Jung
Yang Hyun-Won
Suh Hyung-Joo
Choi Hyeon-Son
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Abstract
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This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.
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KEYWORD
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Human monocyte (THP-1), Red ginseng extract (RGE), Monocyte-to-macrophage differentiation, Inflammatory signaling
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